Un-Answered Problems With Online Research Paper Writercustom Written Research Paper Exposed

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Biochemical Techniques for the Extraction of Escherichia...

Modern biochemical study and analysis of nucleic acids have been heavily dominated by electrophoresis and polymerase chain reaction techniques, as the former allows for relatively inexpensive and accessible resolution and visualization of nucleic acids according to basic chemical properties such as molecular charge and weight, and the latter quickly increases the concentration of nucleic acids, normally found in cells in minute amounts, to a level easily analyzed by modern biochemical techniques. These two techniques are therefore currently indispensable in dealing with nucleic acids on a practical level, and are tools which should be present in every biologist’s kit. This study therefore attempts to elucidate the theoretical and†¦show more content†¦While ample plant and animal samples can be easily obtained, for bacterial samples such as E. coli, they must be propagated first in culture in order to obtain the necessary amount of cells needed for the DNA extraction p rocess. The extraction and purification of genomic DNA is essential as purified DNA serves as the starting point for the amplification of a gene within the DNA via the Polymerase Chain Reaction (PCR). First and foremost, cell lysis or cell disruption must be done to release the cell contents to expose the DNA within the organism, detergents, basic or high salt solutions will then be used for DNA purification by dissociating other biomolecules such as proteins and lipids. DNA will finally be isolated through the use of organic reagents and centrifugation to facilitate collection of the precipitated DNA. Prior to the experiment, materials to be used were sterilized to prevent contamination, which may later on affect the results as contaminants may react with the solutions to be used for the later procedures or even damage the DNA samples. Autoclaving or the use of pressurized steam was utilized to sterilize the materials as exposure to high temperatures normally causes damage to cytoplasmic membranes, breakdown of ribosomes, irreversible enzyme denaturation and DNA strand breakage of bacterial contaminants. Due to the ability of nucleic acids to store genetic information which will later on encode for necessary proteins, it is important to extractShow MoreRelatedA summary analysis of the article â€Å"Microarray detection of food-borne pathogens using specific probes prepared by comparative genomics.†1531 Words   |  7 Pagesthe most common food-borne pathogens that maybe seen in the United States are Norvovirus (58%), Clostridium perfringens (10%), Salmonella (11%), Campylobacter spp. (9%), and Staphylococcus aureus (3%). Among the other 9% (not published) include Escherichia coli O157:H7, Shigella spp., Bacillus spp., and other opportunistic pathogens (Prasad Vidyarthi, 2009). Therefore, accurate and timely detection and identification of food-borne pathogens is crucial for the prevention of food-borne epidemics and

Differences between Japan and American free essay sample

With the development of globalization, the commercial activities between East and West has become one of the major lifeline almost dominated the world economy. Its representation can be attributed to the Japanese and U. S. economic and trade. As is well known, the United States is the superpower of the world, not only in military but also in economic. Japan is a small country, while considering the contribution to the world economic Japan still is a big country. So trade exchanges and business negotiations between these two countries is a systematic project because of different national culture, beliefs, values and norms. All of these differences may lead to some unpredictable issues in commercial activities if both side do not clearly know the difference. Japan is a typical oriental culture nurtured the country, while the United States is a combination of Western civilization itself nurtured the development of the United States set their own unique culture. These two contradictory business negotiations and cultural differences between countries and thus caused inconvenience to bring the negotiation process was affecting each other. Therefore, this progress report is going to discuss the differences between Japan and American, and try to give some suggested recommendations aimed to solve the problems in commercial activates. 2. Differences between Japan and American 2. 1 national culture difference As is known, Japan is an island country, ancient time was influenced by Chinese Confucian thought, formed a national unique isolation from outside environment. In the face of external human isolation, a self-defence will become more and more obvious and sensitive. It is in this case that they need to use a polite but not offend traditional methods of others to communicate with people. However, American history, it was only a few hundred years, but as one of the worlds largest nation of immigrants, including the formation of the cultural history of the United States can be traced to the history of the West. The original owner or the United States is the ruler over the Westerners from Europe. Facing of such a huge development space, they form a rich cultural patterns of young passion. These two different national culture were caused by history issues and may remain for a long period in the future. The emergence of conflicts is inevitable, and reverse this way of thinking is also unavoidable in different culture come together. The difference will be introduced in detail in the final report. 2. 2 communication and negotiation style differences These two countries have totally reverse styles in communication and commercial negotiation. To begin with, American businessmen are free to negotiate the rapid negotiation outcome. They do not pay attention to position, title and etiquette, the negotiation process does not focus on body language and posture, quickly cut to the chase. On the contrary, the Japanese negotiations nature is a very long time. They like to invite guests to drink tea several times before entering the topic. So that they can understand each others ins and outs, like jobs, interests, background and so on. Japanese businessmen are typical Asian style, they are more cautious than people in other countries, strong, patient, confident, motivated and active. They do not easily show their attitude during the negotiations. Americans like to use humour in the negotiation process, but the Japanese are polite and courteous. When they disagree, Japanese always keep silence and smile. Even the other party has lost his temper, Japanese still keep smiling. In the eyes of Westerners, this is rude, and even contempt for himself, and Japanese is just trying to show the gentleman. All of these communication differences will be associated with specific examples in final report. 2. 3 differences in Business Etiquette Business etiquette is an important aspects in commercial activities, and Japan has a different etiquette compared with American. To begin with, Americans often is nodded smiling, polite to say hello on the line, even at the first sight, not necessarily shake hands; and whether they are young or old, people often call them by their names. However, Japanese usually meet will greet each other, bowing, 15 degrees is the general etiquette, and 30 degrees for the common courtesy, 45 degrees is the most respected etiquette. If it is an acquaintance or friend, can take the initiative to shake hands or hug. Meet for the first time to do a 90 degree bow, mens hands hanging down with legs bowed, lady left hand pressure in the right hand on the lower abdomen before the bow, parallel read ‘the first meeting, please’. At the same time, the Japanese name and title is also very important, they like to call each other title without name. Secondly, changing business card is a significant process in commercial negotiation. Also, differences will occur during this process. Americans usually dont give each other cards, unless you want to keep in touch with each other. On the contrary, send business cards in Japan is very popular and common thing. Japanese businessmen meeting for the first time when the exchange of business cards. Usually, the young man or low status people will take the initiative to submit a business card to people of high status than their older or watching each other with respect of the eyes. But if both sides are not prepared card, they will take the initiative to the other simple self-introduction. Thus the differences in business etiquette are significant in commercial activities. 3. Suggested recommendations The way to solve the differences is to respect each others way of thinking and cultural impacts. Try to use each others way of thinking to explore the issue and the parties concerned to solve the problem involved in business negotiations. For instance, when Japanese communicate to Americans in the project, they ran out into the theme of the book directly, without veiled expression of his intention; In contrast, Americans in the project is the first book on the Japanese greeting his speech, and then slowly brewing an atmosphere, and finally the expression was suddenly intent. Thus, the both parties involved and had a smooth negotiation. Unpleasant thinking upside down on their cultural influence would not be produced, the contradiction between the two sides will eliminate or unpleasant. Good atmosphere will form a business negotiation, respect for each other will form a cooperative atmosphere. Secondly, Americans personality exposed, passionate, they believe that mutual tolerance in order to break the deadlock, willing to make concessions in the negotiations, the negotiations proceed smoothly. Americans pay attention to efficiency, shorten the negotiation time. In the process of negotiation to negotiation, they like a fact and a fact, one question after another discussion, finally complete the entire agreement. Thirdly, negotiations with the Americans coping skills. With American negotiations should have enthusiasm, confidence, to create a good atmosphere of negotiations, win. Negotiations with the Americans, the characteristics of attention should be paid to Americans rich for a supply of sth. and interested in package transactions, active use of the other forces to promote cooperation, a wide range of global balance, a package deal. At the same time, negotiations with the Americans must be punctual, efficient service to. 4. Conclusion When two very different cultural patterns that appear in the same place when the inevitable conflicts arise there. But in order to make way both to have survived a virtuous cycle, which means a win-win model, and thus will not be swallowed up or assimilated. Then you need both sides to respect each others culture conscious, respect each others thinking. On this basis, and learn each others essence, to its dregs, they belong to the cultural progress of sublimation. Upside of thinking certainly desirable, but it is also a historical period in ways that are bound to exist. In order to improve this negative cycle of communication, is that both sides need the efforts of the two sides can come up with is reversed brave enough to deal with, so will understand each others true intentions, mutual respect and harmonious development will be more exchanges with cooperation. All in all, the difference is there and what we need to do in commercial activities is to find it, realise it, and try to overcome it. Reference

Why the Drinking Age Should Not Be Lowered Essay Example Essay Example

Why the Drinking Age Should Not Be Lowered Essay Example Paper Why the Drinking Age Should Not Be Lowered Essay Introduction Heather Evans Mr. PhillipsEnglish 10211/25/2010 | | Why the Drinking Age Should Not be Lowered. | | According to the U. S. Department of Health and Human Services, â€Å"Increasing the age at which people can legally purchase and drink alcohol has been the most successful intervention to date in reducing drinking and alcohol-related crashes among people under the age of 21. †(Nakaya) Among teens and young adults, alcohol is the drug of choice. Many are not aware that they are harming their bodies when drinking. Teens may come intoxicated and make harmful decisions that they would not normally make when not under the influence. Many young people are experiencing the consequences of drinking too much, at too early an age. As a result, underage drinking is a leading public health problem in this country (Ojeda). According to the Department of Health and Human Services, approximately 5,000 young people under the age of 21 die as a result of underage drinking. Some are crash relate d, homicide, suicide, and other injuries such as falls, burns, and drownings. When President Ronald Reagan signed this law on July 17, 1984 at the White House Rose Garden ceremony, he said, â€Å"We know that people in the 18-to-20 age group are more likely to be in alcohol-related accidents than those in any other age group. We know that America has a clear stake in making certain that her sons and daughters, so full of vitality and promise, will not be crippled or killed. Why the Drinking Age Should Not Be Lowered Essay Body Paragraphs And we all know that there is one simple measure that will save thousands of young lives if we raise the drinking age to 21†(Hamilton). Teens are starting to drink earlier each year. In 1965, the average drinking age was around 17 1/2. In 2003, the average drinking age was around 14. By lowering the age to drink, people are giving teens and young adults permission to harm themselves and possibly harm others. The brain keeps developing in to the twenties so if teens drink at the age of 18 or younger they are not allowing the brain to function correctly. Auriana Ojeda states that â€Å"Differences between the adult brain and the brain of the maturing adolescent also may help to explain why many young drinkers are able to consume much larger amounts of alcohol than adults before experiencing the negative consequences of drinking, such as drowsiness, lack of coordination, and withdrawal/hangover effects. This unusual tolerance may help to explain the high rates of binge drinking a mong young adults. At the same time, adolescents appear to be particularly sensitive to the positive effects of drinking, such as feeling more at ease in social situations, and young people may drink more than adults because of these positive social experiences. † Young people that start using alcohol at an early age are more prone to use alcohol when they are older. This may also result in binge drinking and alcoholism. Why do some teens and young adults drink? No one really knows the answer to that but it has become clear that some do it because of peer pressure. A teen goes to a party and sees that all of their friends are drinking, so he or she decides that they want to fit in and follow what their friends are doing. This also leads to other drug usage. Peer pressure is one of the main sources of underage drinking. Another reason teens may drink is that they may see their parents drinking and see that it may be fun. Drinking is not always fun. Some adults know how to act w hen they drink and are responsible enough to know when to stop. They also might call someone to come get them when they have been drinking. Teens may not call someone. They see that it will be â€Å"cool† if they drink and drive not realizing the risks of it. Also adults that drink more may have children who favor drinking and will drink a lot. Radio, television, and the internet also have a big part on teenage drinking. Young adults see movies and TV shows that promote underage drinking and it makes drinking seem fun and not risky. They do not realize that it is just television and some of the things that are done are done by professionals and stunt people. And normally they are not drinking in the shows. Just making it seem like it. The media uses this to their advantage. The alcohol companies pay the media to do commercials and the media pay the companies to use their product in shows and movies. Alcohol commercials are now made to where they make the alcohol desirable to young people so they can sell more. Stores that provide alcohol are accepting to these commercials because it allows them to make more money. It does not matter to them if the adult gives the alcohol to young teens. As long as the teen does not buy it they do not care. Raising the price of alcohol may also help out underage drinking. Ojeda also states in his research that teens also do not see the health risks that they are putting themselves in. They are more prone to liver damage, obesity, and other major health issues. Studies show that with teens that are overweight or obese, they have elevated liver enzymes even with only moderate drinking. Teens that are going through or about to go through puberty also show the upset of the critical hormonal balance necessary for normal development of organs, muscles, and bones. Ojeda) Studies in animals show that drinking during puberty also has an effect on the reproductive system. Other risky behaviors may include unplanned sexual activity . This may result in an unplanned pregnancy or forms of STD. Teens are more hormonal and with the influence of alcohol they may make decisions that will affect the rest of their life. They may also develop an alcohol use disorder. Young people who drink heavily are also more prone to drinking heavily as adults which may cause health issues as they get older. Having the drinking age set to 21 years of age has been a proven success. An estimated 20,000 lives have been saved due to the law. (Hamilton) Since President Reagan established the law, the fatal accidents involved with drunk driving or young people that have been drinking and were in an accident, has been cut in half. That does not even include the number of injuries that have been reduced by the law. The â€Å"21† law is very effective. It does not stop teens from drinking but it does stop them from being able to buy the alcohol. Removing the law would help the companies and the stores that sell alcohol, but it will al so make the number of deaths rise and the number of young adults that drink heavily rise as well. If people remove the â€Å"21† law they might as well let drugs be legal. Alcohol is just a drug for young teens to experience. Other consequences of underage drinking include death from alcohol poisoning, academic problems, various social problems, and physical problems such as medical illnesses. (Nakaya) Teens that consume alcohol on a daily basis or quite often experience changes in school and other things that they normally would do. Underage drinking may result in teens losing friendships over it, failing grades, and also becoming ill more often in a result of their immune system not fully in order. Teens may feel like they are not accepted in society so they drink more to help them with the emptiness. They may start to think that their parents are always yelling or getting on to them or they are always in trouble because they are in a state of mind where they cannot functio n without the alcohol. Once they consume some, teens are back to the normal state of mind in their eyes and can function. Because of this teens are getting addicted and become very dependent on alcohol. If they do not get their alcohol they go into a depression and start having withdraws. At this time they should realize what they are doing to their bodies but some do not and they continue to drink and harm themselves. College students may also be more affected by alcohol because teens see that college is just one big party. Teens that are just enrolling in college are trying to fit in. They go to parties and drink heavily. They then become intoxicated and are more accepting to do dumb things. A college student is new to the environment and does not know how to act. Some students are focused on school and do not go to parties and if they do they do not drink. The students that do drink feel that they are accepted in the society of college and may think it is the right path for them. They become overwhelmed and most do not finish out the degree program. Some realize what they are doing and stop drinking and become more academically focused and succeed in getting their degree. As you can see there are many reasons on why the drinking age should not be lowered. I feel that people between the ages of 18 – 21 are not fully aware of all the risks and may have alcohol as a crutch. Teens need to focus on school and doing something with their life. There are too many people in the world that have let alcohol consume them and have not done anything with they have wanted. Works Cited: Hamilton, Wendy J. â€Å"Age-Twenty-One Drinking Laws Protect Youth. † Alcohol. Ed. Andrea C. Nakaya. Detroit: Greenhaven Press, 2008. Opposing Viewpoints. Nakaya, Andrea C. U. S. Department of Health and Human Services. â€Å"Underage Drinking Is a Serious Problem. † Alcohol. Ed. Detroit: Greenhaven Press, 2008. Nelson, David E. â€Å"The Drinking Age Should Not Be Lo wered. † Teen Drug Abuse. Detroit: Greenhaven Press, 2011. Opposing Viewpoints. Ojeda, Audriana. U. S. Department of Health and Human Services. â€Å"The Minimum Legal Drinking Age Should Not Be Lowered. † Teens at Risk. Ed. San Diego: Greenhaven Press, 2009. We will write a custom essay sample on Why the Drinking Age Should Not Be Lowered Essay Example specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Why the Drinking Age Should Not Be Lowered Essay Example specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Why the Drinking Age Should Not Be Lowered Essay Example specifically for you FOR ONLY $16.38 $13.9/page Hire Writer